MBL-C antibody

• Catalog Number: orb1109169
• Category: Antibodies
• Description: Mouse monoclonal antibody to MBL-C
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IA, IF, IHC-Fr, WB
• Reactivity: Mouse
• Isotype: Rat IgG2a
• Form/Appearance: 1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
• Conjugation: Unconjugated
• Storage: Product should be stored at 4degC. Under recommended storage conditions, product is stable for one year.
• Note: For research use only
• Application notes: F: Cryostat sections (7um) of mouse tissue were acetone fixed and stained o/n at 4degC in TBS/2% goat serum. (Ref.4); IA: Biotinylated 14D12 was used as detector in ELISA at 1µg/ml TBS/Tween-20/Ca (Ref.1); IF: Hyphae of C. albicans were fixed on poly-l-lysin coated slides with ice-cold aceton. Slides were incubated for 1h at RT with 14D12. (Ref.3); W: On a 12% non-reducing SDS-PAGE a prominent band of ca. 50Kda is detected. Under reducing conditions a 26 KDa band is detected. (Ref.2); MBL-C (clone 14D12) deposition in developing murine atherosclerotic lesions following 10 weeks of high fat feeding. MBL-C was detected in and around invading macrophages invading the intima (insert). MBL-C bound, similar to MBL-A, at sites of necrosis (upper right corner). No MBL-C binding was shown in the media or on fibrous caps covering the thickened intima.
• Expiration Date: 12 months from date of receipt.