MAT2A Antibody

• Catalog Number: orb1523289
• Category: Antibodies
• Description: MAT2A Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 1 and 50
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:10,000 - 1:25,000; IP - 2-10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: MAT2A
• UniProt ID: P31153
• NCBI: NP_005902.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: methionine adenosyltransferase 2; methionine adeno
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse MAT2A by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MAT2A antibody (orb1523289) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Detection of human MAT2A by western blot. Samples: Whole cell lysate (10 µg) from HeLa, HEK293T, Jurkat, Hep-G2, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MAT2A antibody (orb1523289) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human MAT2A by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MAT2A antibody (orb1523289) used for IP at 6 µg per reaction. MAT2A was also immunoprecipitated by a previous lot of this antibody (orb1523289) and a second antibody against a different epitope of MAT2A.