Mast Cell Tryptase/TPSAB1 Antibody

• Catalog Number: orb371750
• Category: Antibodies
• Description: Mast Cell Tryptase/TPSAB1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human Mast Cell Tryptase recombinant protein (Position: H65-P275). Human Mast Cell Tryptase shares 77% and 76.1% amino acid (aa) sequence identity with mouse and rat Mast Cell Tryptase, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat ELISA(Cap) , 1-5μg/ml, Human, -
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 30515 MW
• UniProt ID: Q15661
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Tryptase alpha/beta-1;Tryptase-1;3.4.21.59;Tryptas
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of Mast Cell Tryptase using anti-Mast Cell Tryptase antibody.Lane 1:293T cell.

Sandwich ELISA - Recombinant human Tryptase/TPSAB1, B2 protein standard curve.Use in combination with reagents from Human Tryptase/TPSAB1, B2 ELISA Kit EZ-Set (DIY Antibody Pairs).

IHC analysis of Mast Cell Tryptase using anti-Mast Cell Tryptase antibody. Mast Cell Tryptase was detected in a paraffin-embedded section of human lung cancer tissue.