Mast Cell Tryptase Recombinant Rabbit Monoclonal Antibody

SKU: orb612206

Description

Mast Cell Tryptase Recombinant Rabbit Monoclonal Antibody

Research Area

Amino Acid Metabolism, Cancer Research, Inflammation, Macrophage

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-5000, IHC-P=1:200-1000, IHC-F=1:200-1000, IF=1:200-1000
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: B5I5
• Immunogen: A synthesized peptide derived from human Tryptase 1 (10-275/275aa)
• Target: TPSAB1
• Molecular Weight: 33 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TPS1; TPS2; TPSB1; TPSB2; Tryptase-2; MMCP-7; Mcp-7; Mcp7; Mcpt7; rMCP-7; TRYB1_HUMAN; TPSAB1; Tryptase-1; Tryptase I; Tryptase alpha-1; 3.4.21.59; TRYB1_MOUSE; Mast cell protease 7 (mMCP-7); Tryptase alpha/beta-1; TRYB1_RAT; Mast cell protease 7 (rMCP-7); Tryptase, skin; TRYB2_HUMAN; Tryptase II;

Paraformaldehyde-fixed, paraffin embedded (human Abdominal skin), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Mast Cell Tryptase) Monoclonal Antibody, Unconjugated (orb612206) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human coion), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Mast Cell Tryptase) Monoclonal Antibody, Unconjugated (orb612206) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human duodenum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Mast Cell Tryptase) Monoclonal Antibody, Unconjugated (orb612206) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Mast Cell Tryptase) Monoclonal Antibody, Unconjugated (orb612206) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Mast Cell Tryptase) Monoclonal Antibody, Unconjugated (orb612206) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Skin tissue lysates, Lane 2: Mouse Stomach tissue lysates, Primary: Anti-Mast Cell Tryptase (orb612206) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 23 kDa, Observed band size: 32 kDa.

Quick Database Links

Gene Symbol

TPSAB1

UniProt

Q15661