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Catalog Number | orb1274518 |
---|---|
Category | Antibodies |
Description | MAPK9 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-424 of human MAPK9 (NP_002743.3). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 54kDa |
Target | MAPK9 |
UniProt ID | P45984 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | MAPK9, stress-activated protein kinase JNK2, SAPK, Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using MAPK9 antibody (orb1274518) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts from normal (control) and MAPK9 knockout (KO) 293T cells, using MAPK9 antibody (orb1274518) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human breast cancer using MAPK9 antibody (orb1274518) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using MAPK9 antibody (orb1274518) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using MAPK9 antibody (orb1274518). Blue: DAPI for nuclear staining.
IF, IH, WB | |
Human, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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