MAPK14 Antibody

• Catalog Number: orb1259967
• Category: Antibodies
• Description: MAPK14 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human p38 MAPK (NP_620581.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:1000IF: 1:50 - 1:100IP: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 41kDa
• Target: MAPK14
• UniProt ID: Q16539
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: MAPK14, RK, p38, CSBP, EXIP, Mxi2, CSBP1, CSBP2, C
• Note: For research use only
• Application notes: WB: 1:500 - 1:1000IF: 1:50 - 1:100IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using p38 MAPK antibody (orb1259967) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunofluorescence analysis of NIH-3T3 cells using p38 MAPK antibody (orb1259967) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of U-87MG cells, using 3 ug p38 MAPK antibody (orb1259967). Western blot was performed from the immunoprecipitate using p38 MAPK antibody (orb1259967) at a dilution of 1:1000.