MAPK12 monoclonal antibody (M01), clone 4F4-3D2

• Catalog Number: orb2292210
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a full length recombinant MAPK12.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 4F4-3D2
• Tested applications: ELISA, IF, WB
• Reactivity: Human
• Isotype: IgG1 kappa
• Immunogen: MAPK12 (AAH15741.1, 1 a.a. ~ 367 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Protein Sequence: MSSPPPARSGFYRQEVTKTAWEVRAVYRDLQPVGSGAYGAVCSAVDGRTGAKVAIKKLYRPFQSELFAKRAYRELRLLKHMRHENVIGLLDVFTPDETLDDFTDFYLVMPFMGTDLGKLMKHEKLGEDRIQFLVYQMLKGLRYIHAAGIIHRDLKPGNLAVNEDCELKILDFGLARQADSEMTGYVVTRWYRAPEVILNWMRYTQTVDIWSVGCIMAEMITGKTLFKGSDHLDQLKEIMKVTGTPPAEFVQRLQSDEAKNYMKGLPELEKKDFASILTNASPLAVNLLEKMLVLDAEQRVTAGEALAHPYFESLHDTEDEPQVQKYDDSFDDVDRTLDEWKRVTYKEVLSFKPPRQLGARVSKETPL
• NCBI: AAH15741.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Detection limit for recombinant GST tagged MAPK12 is approximately 1 ng/ml as a capture antibody.

Immunofluorescence of monoclonal antibody to MAPK12 on HeLa cell. [antibody concentration 10 ug/ml]

Western Blot analysis of MAPK12 expression in transfected 293T cell line by MAPK12 monoclonal antibody (M01), clone 4F4-3D2. Lane 1: MAPK12 transfected lysate (41.9 KDa). Lane 2: Non-transfected lysate.

Western Blot detection against Immunogen (66 KDa).