MAPK1 Antibody

• Catalog Number: orb169874
• Category: Antibodies
• Description: Mouse Monoclonal antibody to MAPK1.Mitogen-activated protein kinase 1 (MAPK1) is also known as extracellular signal-regulated kinase 2 (ERK2). Two similar (85% sequence identity) protein kinases were originally called ERK1 and ERK2. They were found during a search for protein kinases that are rapidly phosphorylated after activation of cell surface tyrosine kinasessuch as the epidermal growth factor receptor. Phosphorylation of ERKs leads to the activation of their kinase activity. The molecular events linking cell surface receptors to activation of ERKs are complex. It was found that RasGTP-binding proteins are involved in the activation of ERKs. Another protein kinase, Raf-1, was shown to phosphorylate a MAPK kinase, thus qualifying as a MAPK kinase kinase. The MAPK kinase was named MAPK/ERK kinase (MEK). Receptor-linked tyrosine kinases, Ras, Raf, MEK and MAPK could be fitted into a signaling cascade linking an extracellular signal to MAPK activation. Transgenic gene knockoutmice lacking MAPK1 have major defects in early development.
• Clonality: Monoclonal
• Isotype: Mouse IgG2b heavy chain and κ light chain
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
• Immunogen: Anti-human MAPK1 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human MAPK1 amino acids 1-360 purified from E. coli
• Application notes: Applications: MAPK1 antibody has been tested by ELISA, Western blot and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:500 ~ 3000.Recommended starting dilution is 1:500
• Clone Number: PAT1A4AT
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Mitogen-activated protein kinase 1, EC 2.7.11.24,
• Note: For research use only