MAP3K8 Antibody

• Catalog Number: orb371741
• Category: Antibodies
• Description: MAP3K8 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human MAP3K8 recombinant protein (Position: D9-L193). Human MAP3K8 shares 89.7% and 90.3% amino acid (aa) sequence identity with mouse and rat MAP3K8, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 5μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 52925 MW
• UniProt ID: P41279
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Mitogen-activated protein kinase kinase kinase 8;2
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U937 cells using anti-MAP3K8 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of MAP3K8 using anti-MAP3K8 antibody.Lane 1:human HEPG2 cell; 2:human HELA cell; 3:human A431 cell; 4:human A549 cell; 5:human THP-1 cell; 6:human Daudi cell; 7:rat liver tissue; 8:mouse liver tissue.

IF analysis of MAP3K8 using anti-MAP3K8 antibody. MAP3K8 was detected in immunocytochemical section of HepG2 cells.

IHC analysis of MAP3K8 using anti-MAP3K8 antibody. MAP3K8 was detected in paraffin-embedded section of rat brain tissue.

IHC analysis of MAP3K8 using anti-MAP3K8 antibody. MAP3K8 was detected in paraffin-embedded section of mouse brain tissue.

IHC analysis of MAP3K8 using anti-MAP3K8 antibody. MAP3K8 was detected in paraffin-embedded section of human lung cancer tissue.

IHC analysis of MAP3K8 using anti-MAP3K8 antibody. MAP3K8 was detected in paraffin-embedded section of human pancreatic cancer tissue.

IHC analysis of MAP3K8 using anti-MAP3K8 antibody. MAP3K8 was detected in paraffin-embedded section of human rectal cancer tissue.