MAP3K7 Antibody

• Catalog Number: orb1246877
• Category: Antibodies
• Description: MAP3K7 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, WB
• Predicted Reactivity: Bovine, Rat
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: C-AELDQDEKDQQNT
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 75kDa band observed in lysates of cell lines HeLa, Daudi and NIH3T3, and approx. 80kDa in lysates of cell line U937 (calculated MW of 67.2 according to Human NP_663304.1 and Mouse NP_033342.1). The observed molecular weight corresponds to different antibodies from other commercial sources. Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: MAP3K7
• UniProt ID: O43318
• NCBI: NP_663304.1, NP_003179.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: MAP3K7 , TAK1, mitogen-activated protein kinase ki
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 75kDa band observed in lysates of cell lines HeLa, Daudi and NIH3T3, and approx. 80kDa in lysates of cell line U937 (calculated MW of 67.2 according to Human NP_663304.1 and Mouse NP_033342.1). The observed molecular weight corresponds to different antibodies from other commercial sources. Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246877 (1 ug/ml) staining of HeLa (A) and U937 (B) and (2 ug/ml) Daudi (C) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246877 (2 ug/ml) staining of NIH3T3 cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246877 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).