MAP2K5 antibody

• Catalog Number: orb378166
• Category: Antibodies
• Description: Rabbit polyclonal antibody to MAP2K5.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full length protein of human MKK5
• Dilution range: WB: 1:500-2000, IF/ICC: 1:50-100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: MAP2K5
• Entrez: 5607, 29568, 23938
• UniProt ID: Q9WVS7, Q13163, Q62862
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: MEK5; MKK5; PRKMK5; Dual specificity mitogen-activ
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of MKK5 expression in H460 (A), BT474 (B), mouse liver (C), rat kidney (D) whole cell lysates. (Predicted band size: 46; 48; 49; 50 kD; Observed band size: 50 kD)

Immunohistochemical analysis of MKK5 staining in rat heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of MKK5 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.