MAP2K4 Antibody

• Catalog Number: orb1256231
• Category: Antibodies
• Description: MAP2K4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide of human MAP2K4
• Concentration: batch dependent
• Dilution range: WB: 1:1000 - 1:4000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 44kDa
• Target: MAP2K4
• UniProt ID: P45985
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: MAP2K4, JNK activating kinase 1, JNK-activated kin
• Note: For research use only
• Application notes: WB: 1:1000 - 1:4000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using MAP2K4 antibody (orb1256231) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Immunohistochemistry of paraffin-embedded human kidney using MAP2K4 antibody (orb1256231) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using MAP2K4 antibody (orb1256231) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of MCF-7 cells, using 3 ug MAP2K4 antibody (orb1256231). Western blot was performed from the immunoprecipitate using MAP2K4 antibody (orb1256231) at a dilution of 1:1000.