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Catalog Number | orb1294406 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to MAP 2 |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rabbit |
Isotype | Rabbit-IgG |
Immunogen | Synthetic peptide / encompassing a sequence within the N-terminus region. |
Dilution range | Western Blot 1:500-1:1000 Immunofluorescence 1:200-1:300 Immunohistochemistry (Paraffin) 1:100-1:150 |
Conjugation | Unconjugated |
Target | MAP2 |
Storage | Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
All lanes: Anti-MAP2 antibody at 1/500 dilution, Lysates/proteins at 60 μg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294406 overnight at 4°C.
Immunofluorescent analysis. Sample: primary cortical neurons; MAP2 antibody (orb1294406): 1-200; Anti-rabbit 488: 1-500; Fixed: 4% paraformaldehyde at RT for 20 min.
Immunohistochemical analysis of paraffin embedded Human brain and mouse tissue labeling MAP2 antibody with orb1294406 at 1/100.
Immunohistochemical analysis of paraffin embedded Human brain and mouse tissue labeling MAP2 antibody with orb1294406 at 1/100.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294406 anti-MAP2 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
FC, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IHC-P | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
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