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Catalog Number | orb389425 |
---|---|
Category | Antibodies |
Description | Mannose Phosphate Isomerase/MPI Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E. coli-derived human MPI recombinant protein (Position: A2-K99). Human MPI shares 88.8% and 86.7% amino acid (aa) sequence identity with mouse and rat MPI, respectively. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Flow Cytometry, 1-3μg/1x106 cells, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 46656 MW |
UniProt ID | P34949 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Mannose-6-phosphate isomerase;5.3.1.8;Phosphohexom Read more... |
Note | For research use only |
Application notes | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of A549 cells using anti-MPI antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of MPI using anti-MPI antibody.Lane 1:human HeLa cell;2:human Caco-2 cell;3:human HEK293 cell;4:rat brain tissue;5:rat lung tissue;6:rat heart tissue;7:mouse brain tissue;8:mouse lung tissue.
IHC analysis of MPI using anti-MPI antibody.MPI was detected in paraffin-embedded section of human intestinal cancer tissues.
IHC analysis of MPI using anti-MPI antibody.MPI was detected in paraffin-embedded section of human lung cancer tissues.
IHC analysis of MPI using anti-MPI antibody.MPI was detected in paraffin-embedded section of rat brain tissue.
FC, IHC, WB | |
Human, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, WB | |
Human, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, IF, IHC, WB | |
Human, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, IHC, WB | |
Human, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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