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Catalog Number | orb1252044 |
---|---|
Category | Antibodies |
Description | MAGEA1 Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | MAAZ2 |
Tested applications | IF, IHC-P |
Reactivity | Human |
Isotype | IgG1, kappa |
Immunogen | Recombinant human MAGEA1 protein was used as the immunogen for the MAGE-1 antibody. |
Concentration | 0.2 mg/mL |
Dilution range | Immunofluorescence: 1-2 ug/mlImmunohistology (FFPE): 1-2 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the MAGE-1 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | MAGEA1 |
UniProt ID | P43355 |
Storage | Aliquot and Store at 2-8°C. Avoid freez-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Alternative names | Melanoma-associated antigen 1, Antigen MZ2-E, Canc Read more... |
Note | For research use only |
Application notes | Immunofluorescence: 1-2 ug/mlImmunohistology (FFPE): 1-2 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the MAGE-1 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
Formalin-fixed, paraffin-embedded human testicular carcinoma stained with MAGE-1 antibody (MZ2E/838).
IF, IHC, WB | |
Human | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
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