MafA Antibody

• Catalog Number: orb1519834
• Category: Antibodies
• Description: MafA Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: ICC, IHC, WB
• Reactivity: Human, Mouse
• Immunogen: between 125 and 175
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:1000; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Zinc-fixative (JB fix) is recommended to enhance staining.; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; IHC-IF - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Zinc-fixative (JB fix) is recommended to enhance staining.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: MafA
• UniProt ID: Q8NHW3
• NCBI: NP_963883.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Alternative names: v-maf avian musculoaponeurotic fibrosarcoma oncoge
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse MafA by western blot. Samples: Whole cell lysate (10 µg) from Beta-TC-6, AlphaTC1 Clone 9, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MafA recombinant monoclonal antibody (orb1519834) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human MafA by western blot. Samples: Whole cell lysate (10 µg) from HEK293T transfected with myc tagged Human MafA, Human MafB, and Human cMaf prepared using NETN lysis buffer. Antibody: Rabbit anti-MafA recombinant monoclonal antibody (orb1519834) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit recombinant monoclonal antibody to Myc-tag.

Detection of mouse MafA by western blot. Samples: Whole cell lysate (10 µg) from HEK293T transfected with myc tagged mouse MafA, Human MafB, and Human cMaf prepared using NETN lysis buffer. Antibody: Rabbit anti-MafA recombinant monoclonal antibody (orb1519834) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit recombinant monoclonal antibody to Myc-tag.

Detection of mouse MafA by immunohistochemistry. Sample: FFPE section of mouse pancreas (JB fixation). Antibody: Rabbit anti-MafA recombinant monoclonal antibody (orb1519834). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human MafA by immunohistochemistry. Sample: FFPE section of human pancreatic tumor. Antibody: Rabbit anti-MafA recombinant monoclonal antibody (orb1519834). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse MafA by immunohistochemistry. Sample: FFPE section of mouseBeta-TC-6 cells. Antibody: Rabbit anti-MafA recombinant monoclonal antibody (orb1519834). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human MafA (red) by immunohistochemistry. Sample: FFPE section of human pancreatic carcinoma. Antibody: Rabbit anti-MafA recombinant monoclonal antibody (orb1519834) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).