MADD Antibody

• Catalog Number: orb1527139
• Category: Antibodies
• Description: MADD Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP
• Reactivity: Human
• Immunogen: between 1597 and 1647
• Concentration: 1000 µg/ml
• Dilution range: WB - Not recommended. Use rabbit anti-MADD antibody A302-143A.; IP - 2 - 5 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; IHC-IF - 1:500 - 1:2,500
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: MADD
• UniProt ID: Q8WXG6
• NCBI: NP_003673.3
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: differentially expressed in normal and neoplastic
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human MADD by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MADD antibody orb1527139 used for IP at 3 µg/mg lysate.

Detection of human MADD by immunohistochemistry.Sample: FFPE sections of human breast carcinoma and colon carcinoma. Antibody: Affinity purified rabbit anti-MADD (Cat. No. orb1527139) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB and Red-fluorescent goat anti-rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1: 100