MAD2L1 antibody

• Catalog Number: orb345434
• Category: Antibodies
• Description: MAD2L1 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to N-terminal region near amino acid residues 1-25 of Human MAD2L1 protein.
• Concentration: 1.19 mg/mL
• Dilution range: ELISA: 1:2,000 - 1:10,000, IF: User Optimized, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This affinity purified antibody is directed against human MAD2L1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human, dog, macaque, chimpanzee and gecko based on 100% homology for the immunogen sequence. Cross reactivity with MAD2L1 may occur from mouse and chicken sources, as only a two amino acid residue change is found within the immunogen sequence (90% positive by BLAST). Cross reactivity with MAD2L1 homologues from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: Q13257
• NCBI: 4505067
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-MAD2L1 antibody, MAD2, MAD2-like prote
• Note: For research use only
• Application notes: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~ 24 kDa corresponding to full length protein by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Jurkat (lane 1) and HeLa (lane 2) whole cell lysates using MAD2L1 antibody