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Catalog Number | orb1530390 |
---|---|
Category | Antibodies |
Description | MAD2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP |
Reactivity | Human |
Immunogen | between 100 and 150 |
Concentration | 1000 µg/ml |
Dilution range | WB - Not recommended. Use rabbit anti-MAD2 antibody A300-301A.; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | MAD2 |
UniProt ID | Q13257 |
NCBI | NP_002349.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | MAD2; mitotic arrest deficient 2-like protein 1; m Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human MAD2 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MAD2 used at a dilution of 1:5, 000 (0.2µg/ml).
Detection of human MAD2 by western blot of immunoprecipitates.Samples: Whole cell lysate from HEK293T cells. Antibodies: Affinity purified Rabbit anti-MAD2 antibody.
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