LY96 Antibody

• Catalog Number: orb1239627
• Category: Antibodies
• Description: LY96 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Anti-MD-2 antibody (orb1239627) was raised against a peptide corresponding to 13 amino acids near the center of human MD-2. The immunogen is located within the last 50 amino acids of MD-2.
• Concentration: 1 mg/mL
• Dilution range: WB: 0.5-1 μg/mL; IHC: 2-2.5 μg/mL; IF: 10-20 μg/mL.Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human and rat samples; Immunofluorescence in human and rat samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 19kDObserved: 26 kD (Post-modification: 2 N-linked glycosylations)
• Target: LY96
• UniProt ID: Q9Y6Y9
• NCBI: NP_056179
• Storage: MD-2 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: MD-2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: MD-2 Antibody: MD2, MD-2, ly-96, ESOP-1, ESOP1, MD
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot Validation with Recombinant Protein. Loading: 30 ng of human MD-2 recombinant protein per lane. Antibodies: MD-2, orb1239627 (Lane 1: 0.5 µg/mL, Lane 2: 1 µg/mL and Lane 3: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Daudi Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: MD-2, orb1239627 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Mouse Spleen Tissue Lysate. Loading: 15 µg of lysates per lane. Antibodies: MD-2, orb1239627 (Lane 1: 0.5 µg/mL and Lane 2: 1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Mouse Tissue Lysates. Loading: 15 µg of lysates per lane. Antibodies: MD-2, orb1239627 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Rat Spleen Tissue Lysate. Loading: 15 µg of lysates per lane. Antibodies: MD-2, orb1239627 (Lane 1: 0.5 µg/mL and Lane 2: 1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Rat Tissue Lysates. Loading: 15 µg of lysates per lane. Antibodies: MD-2, orb1239627 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry Validation of MD-2 in Human Spleen Tissue. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-MD-2 antibody (orb1239627) at 2.5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of MD-2 in Human Spleen Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen tissue labeling MD-2 with orb1239627 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).

Immunohistochemistry Validation of MD-2 in Rat Spleen Tissue. Immunohistochemical analysis of paraffin-embedded rat spleen using anti-MD-2 antibody (orb1239627) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of MD-2 in Rat Spleen Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed rat spleen tissue labeling MD-2 with orb1239627 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).