Luteinizing Hormone alpha Antibody / hCG alpha

• Catalog Number: orb386018
• Category: Antibodies
• Description: This mAb reacts with a protein of ~13kDa, identified as alpha subunit of Luteinizing Hormone (LH) or Chorionic Gonadotrophin (CG). The protein dimer contains 2 polypeptide units, labeled alpha and beta subunits that are connected by two bridges. The alpha subunits of LH, FSH, TSH, and hCG are identical, and contain 92 amino acids. The beta subunits vary. LH has a beta subunit of 121 amino acids (LHB) that confers its specific biologic action and is responsible for interaction with the LH receptor. This beta subunit contains the same amino acids in sequence as the beta subunit of hCG and both stimulate the same receptor; however, the hCG beta subunit contains an additional 24 amino acids and the hormones differ in the composition of their sugar moieties. LH is synthesized and secreted by gonadotrophs in the anterior lobe of the pituitary gland. In concert with the other pituitary gonadotropin follicle-stimulating hormone (FSH), it is necessary for proper reproductive function. In the female, an acute rise of LH levels triggers ovulation. In the male, where LH has also been called Interstitial Cell-Stimulating Hormone (ICSH), it stimulates Leydig cell production of testosterone. LH is a useful marker in classification of pituitary tumors and the study of pituitary disease.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: LHa/756
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant full-length human LHa protein was used as the immunogen for the Luteinizing Hormone alpha antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (HIER is not recommended for this antibody)
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Luteinizing Hormone alpha antibody is available for research use only.
• UniProt ID: P01215
• Storage: Store the Luteinizing Hormone alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Luteinizing Hormone alpha antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of Human pituitary gland using hCG alpha antibody