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Catalog Number | orb344289 |
---|---|
Category | Antibodies |
Description | Luciferase antibody (Biotin) |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Isotype | IgG |
Immunogen | Luciferase [Photinus pyralis (Firefly)] |
Antibody Type | Primary Antibody |
Concentration | 10.0 mg/mL |
Dilution range | ELISA: 1:80,000 - 1:310,000, WB: 1:8,000 - 1:31,000 |
Form/Appearance | Lyophilized |
Purity | This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Luciferase [Photinus pyralis (Firefly)]. No reactivity is observed against Sea pansy (Renilla reniformis) luciferase. |
Conjugation | Biotin |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | goat anti-Luciferase Antibody biotin conjugation, Read more... |
Note | For research use only |
Application notes | Anti-Luciferase Peroxidase conjugated antibody is produced from Photinus pyralis (Firefly) which produces a green light with a wavelength of 562 nm. Anti-Luciferase Antibody has been tested by ELISA and western blot. Expect a band at approximately 60.7 kDa in size corresponding to Luciferase by western blotting in the appropriate cell lysate or extract. Anti-Luciferase has been assayed against 1.0 ug of Luciferase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:80,000 to 1:310,000 of the reconstitution concentration is suggested for this product. |
Expiration Date | 12 months from date of receipt. |
Rabbit | |
Polyclonal | |
Biotin |
Rabbit | |
Polyclonal | |
Biotin |