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LTA Antibody (Center)
Catalog Number: orb1928466
| Catalog Number | orb1928466 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB21239 |
| Tested applications | FC, IHC-P, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 22297 Da |
| Target | This LTA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-72 amino acids from the Central region of human LTA. |
| UniProt ID | P01374 |
| NCBI | NP_000586.2, NP_001153212.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Lymphotoxin-alpha, LT-alpha, TNF-beta, Tumor necro Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of LTA Antibody (Center) in MDA-MB231 cell line lysates (35 ug/lane). LTA (arrow) was detected using the purified Pab.
Flow cytometric analysis of HepG2 cells using LTA Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with LTA Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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