LRP11 antibody

• Catalog Number: orb215330
• Category: Antibodies
• Description: Rabbit polyclonal antibody to LRP11
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human LRP11. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1-1000, IP: 1-10-1-100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: LRP11
• Entrez: 84918
• UniProt ID: Q86VZ4
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti Low-density lipoprotein receptor-related prot
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of LRP11 expression in A431 (A), HepG2 (B), Jurkat (C) whole cell lysates. (Predicted band size: 53 kD; Observed band size: 49 kD)

Immunoprecipitation of LRP11 from 0.5 mg Jurkat whole cell extract lysate, using 5 ug of Anti-LRP11 Antibody and 50 ul of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10 min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10 min under agitation. Proteins were eluted by addition of 40 ul SDS loading buffer and incubated for 10 min at 70°C; 10 ul of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with Anti-LRP11 Antibody.