You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb546344 |
---|---|
Category | Antibodies |
Description | LONP1/Lon Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, FC, ICC, IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human LONP1/Lon recombinant protein (Position: R137-A320). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells Direct ELISA, 0.1-0.5μg/ml |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 106 kDa |
UniProt ID | P36776 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Lon protease homolog, mitochondrial; LONHs; Lon pr Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of HL-60 cells using anti-LONP1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of LONP1 using anti-LONP1 antibody.Lane 1:human K562 cell;2:human U-87MG cell;3:human PC-3 cell;4:human A549 cell;5:mouse liver tissue;6:mouse kidney tissue;7:mouse SP20 cell.
IF analysis of LONP1 using anti-LONP1 antibody. LONP1 was detected in immunocytochemical section of A431 cells.
IHC analysis of LONP1 using anti-LONP1 antibody.LONP1 was detected in paraffin-embedded section of human lung cancer tissues.
IHC analysis of LONP1 using anti-LONP1 antibody.LONP1 was detected in paraffin-embedded section of human mammary cancer tissues.
IHC analysis of LONP1 using anti-LONP1 antibody.LONP1 was detected in paraffin-embedded section of human rectal cancer tissues.
Human | |
31.25-2000 pg/mL | |
11.9 pg/mL |
IHC, IHC-P, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
Filter by Rating