LMO4 Antibody

• Catalog Number: orb1270611
• Category: Antibodies
• Description: LMO4 Antibody
• Target: LMO4
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Immunogen: This LMO4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-131 amino acids from the Central region of human LMO4.
• UniProt ID: P61968
• MW: 18 kDa
• Tested applications: FC, IF, WB
• Application notes: For IF starting dilution is: 1:10~50For WB starting dilution is: 1:1000For FACS starting dilution is: 1:10~50
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: LIM domain transcription factor LMO4, Breast tumor
• Note: For research use only
• NCBI: P61968

Fluorescent image of A549 cell stained with LMO4 Antibody.A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with LMO4 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml).LMO4 immunoreactivity is localized to Nucleus significantly.

Western blot analysis in mouse spleen tissue lysates (35 ug/lane).

Flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.