LMNB1 Antibody

• Catalog Number: orb1274472
• Category: Antibodies
• Description: LMNB1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 397-586 of human Lamin B1 (NP_005564.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 70kDa
• Target: LMNB1
• UniProt ID: P20700
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: LMNB1, lamin B1, ADLD, LMN, LMN2, LMNB, MGC111419,
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using Lamin B1 antibody (orb1274472) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Western blot analysis of extracts from normal (control) and Lamin B1 knockout (KO) HeLa cells, using Lamin B1 antibody (orb1274472) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat spinal cord using Lamin B1 antibody (orb1274472) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat heart using Lamin B1 antibody (orb1274472) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using Lamin B1 antibody (orb1274472) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using Lamin B1 antibody (orb1274472) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using Lamin B1 antibody (orb1274472) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of C6 cells using Lamin B1 Polyclonal Antibody (orb1274472) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using Lamin B1 Polyclonal Antibody (orb1274472) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.