Liver X Receptor Antibody

• Catalog Number: orb1248615
• Category: Antibodies
• Description: Liver X Receptor Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: CRLQDKKLPPLLSEI
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Western blot: Approx 55kDa band observed in Human, Liver and Kidney lysates and in preliminary testing of Human Spleen lysate (calculated MW of 51.1kDa according to NP_001238863.1). This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on NIH3T3 and Rat for this particular batch. Immunofluorescence:Strong expression of the protein seen in the nuclei of A549 cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: NR1H3; NR1H2
• UniProt ID: Q13133
• NCBI: NP_005684.2, NP_009052.3
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: NR1H3, LXRA, LXR-a, RLD-1, nuclear receptor subfam
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Western blot: Approx 55kDa band observed in Human, Liver and Kidney lysates and in preliminary testing of Human Spleen lysate (calculated MW of 51.1kDa according to NP_001238863.1). This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on NIH3T3 and Rat for this particular batch. Immunofluorescence:Strong expression of the protein seen in the nuclei of A549 cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248615 (1 μg/ml) staining of Human Liver (A) and Kidney (B) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.