liver Arginase/ARG1 Antibody (monoclonal, 2B12)

• Catalog Number: orb654397
• Category: Antibodies
• Description: liver Arginase/ARG1 Antibody (monoclonal, 2B12)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 2B12
• Tested applications: FC, WB
• Reactivity: Human, Monkey, Mouse, Rat
• Isotype: Mouse IgG2b
• Immunogen: E.coli-derived human liver Arginase/ARG1 recombinant protein (Position: E25-D183).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Monkey, Mouse, RatFlow Cytometry, 1-3μ/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 35 kDa
• UniProt ID: P05089
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of liver Arginase/ARG1 using anti-liver Arginase/ARG1 antibody (orb654397). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates, Lane 3: monkey liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-liver Arginase/ARG1 antigen affinity purified monoclonal antibody (Catalog # orb654397) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90502) with Tanon 5200 system. A specific band was detected for liver Arginase/ARG1 at approximately 35KD. The expected band size for liver Arginase/ARG1 is at 35KD.

Flow Cytometry analysis of Jurkat cells using anti-liver Arginase/ARG1 antibody (orb654397). Overlay histogram showing Jurkat cells stained with orb654397 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-liver Arginase/ARG1 Antibody (orb654397, 1μg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (5-10μg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of SiHa cells using anti-liver Arginase/ARG1 antibody (orb654397). Overlay histogram showing SiHa cells stained with orb654397 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-liver Arginase/ARG1 Antibody (orb654397, 1μg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (5-10μg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.