Lipocalin 2/LCN2 Antibody

• Catalog Number: orb312135
• Category: Antibodies
• Description: Lipocalin 2/LCN2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IHC, IHC-Fr, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived rat Lipocalin 2 recombinant protein (Position: Q21-N198). Rat Lipocalin 2 shares 64.4 % and 81.1 % amino acid (aa) sequence identity with human and mouse Lipocalin 2, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Mouse Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Mouse, Rat Flow Cytometry, 1-3μg/1x106 cells, Human ELISA (cap) ,1-5μg/ml, Rat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 22476 MW
• UniProt ID: P30152
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Neutrophil gelatinase-associated lipocalin;NGAL;Al
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A431 cells using anti-Lipocalin 2 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Lipocalin 2 using anti-Lipocalin 2 antibody.Lane 1:Mouse Lung Tissue; 2:Mouse Intestine Tissue.

IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody. Lipocalin 2 was detected in paraffin-embedded section of Rat Spleen Tissue.

IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody. Lipocalin 2 was detected in paraffin-embedded section of Mouse Spleen Tissue.

IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody.Lipocalin 2 was detected in frozen section of rat lung tissue.

IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody.Lipocalin 2 was detected in frozen section of mouse spleen tissue.

IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody.Lipocalin 2 was detected in frozen section of mouse lung tissue.

IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody.Lipocalin 2 was detected in frozen section of rat spleen tissue.