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Catalog Number | orb1535967 |
---|---|
Category | Antibodies |
Description | LIPE/HSL Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC, IHC-P, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | A 14 AA synthetic peptide near the C-Terminus of human Lipe. The immunogen is located within aa 920-970 of Lipe. |
Concentration | 1 mg/ml |
Dilution range | ELISA, IF, IHC, IHC-P (5 µg/ml), WB (0.5 - 1 µg/ml) |
Conjugation | Unconjugated |
Target | LIPE / HSL |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS containing 0.02% Sodium Azide. PBS containing 0.02% Sodium Azide |
Alternative names | LIPE, Hormone-sensitive lipase, LHS, HSL, Lipase, Read more... |
Note | For research use only |
Application notes | Further information: Lipe antibody can be used for detection of Lipe by Western blot at 0.5 - 1 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 85, 90, 110, 118 kDa Observed: 90 kDa |
Expiration Date | 12 months from date of receipt. |
Immunofluorescence of Lipe in Human Lymph Node cells with Lipe antibody at 20 ug/ml.
Western blot of lipe in human lymph node tissue lysate with lipe antibody at (A) 0.5 and (B) 1 ug/ml.