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    LIN28A antibody

    Catalog Number: orb1473669

    DispatchUsually dispatched within 5-10 working days
    $ 413.00
    Catalog Numberorb1473669
    CategoryAntibodies
    DescriptionMouse monoclonal antibody to LIN28A
    Species/HostMouse
    ClonalityMonoclonal
    Tested applicationsIF, IH, WB
    ReactivityHuman
    ImmunogenRecombinant fusion protein of human LIN28A. The exact sequence is proprietary.
    Dilution rangeWB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/10 - 1/50)
    Form/AppearanceMouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    ConjugationUnconjugated
    TargetLIN28A
    Entrez79727
    UniProt IDQ9H9Z2
    SourceMouse
    StorageShipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
    Buffer/PreservativesMouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    Alternative namesCSDD1; LIN28; ZCCHC1; Protein lin-28 homolog A; Li
    Read more...
    NoteFor research use only
    Expiration Date12 months from date of receipt.
    LIN28A antibody

    Western blot analysis of LIN28A expression in LIN28 recombinant protein (A) whole cell lysates. (Predicted band size: 22 kD; Observed band size: 34 kD)

    LIN28A antibody

    Immunohistochemical analysis of LIN28A staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    LIN28A antibody

    Immunofluorescent analysis of LIN28A staining in human testis cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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