LGR5 antibody

• Catalog Number: orb411798
• Category: Antibodies
• Description: Rabbit polyclonal antibody to LGR5
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Mouse, Rat
• Immunogen: Recombinant full length protein of human GPR49
• Dilution range: WB: 1:500-2000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: LGR5
• Entrez: 8549, 14160, 299802
• UniProt ID: O75473, D4AC13, Q9Z1P4
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti-GPR49 antibody, anti-GPR67 antibody, anti-Leu
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of GPR49 expression in A431 (A), mouse brain (B), rat skeletal muscle (C) whole cell lysates. (Predicted band size: 92; 97; 99 kD; Observed band size: 100 kD)

Immunohistochemical analysis of GPR49 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.