Lgals9 Antibody

• Catalog Number: orb1671758
• Category: Antibodies
• Description: Lgals9 Antibody
• Clonality: Recombinant
• Clone Number: RG9-35
• Tested applications: Blocking, FA, FC
• Reactivity: Mouse
• Isotype: IgG kappa
• Immunogen: This antibody was raised by immunising rats with recombinant mouse galectin-9.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: Lgals9
• UniProt ID: O08573
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: ecalectin, Gal-9, galectin 9, Galectin-9, HUAT, le
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed NIH3T3 cells with anti-Galectin 9 antibody RG9-35. Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric r version of RG9-35 (orb1671758) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671758- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.

Flow cytometry using the anti-Galectin 9 antibody RG9-35. NIH3T3 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r1 version of RG9-35 (orb1671758 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.