Lep Antibody (Biotin)

• Catalog Number: orb1272411
• Category: Antibodies
• Description: Lep Antibody (Biotin)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Rat
• Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant rat Leptin (rat Leptin).
• Concentration: batch dependent
• Dilution range: ELISA:Sandwich:To detect Rat Leptin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Rat Leptin (XP-5213) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat Leptin.Western Blot:To detect rat Leptin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat Leptin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Form/Appearance: Lyophilized
• Conjugation: Biotin
• Target: Lep
• UniProt ID: P50596
• NCBI: P50596
• Storage: Leptin antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
• Alternative names: OB, obese, Ob, Leptin, Obesity factor,
• Note: For research use only
• Application notes: ELISA:Sandwich:To detect Rat Leptin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Rat Leptin (XP-5213) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat Leptin.Western Blot:To detect rat Leptin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat Leptin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

To detect Rat Leptin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Anti-Rat Leptin (orb1272412) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat Leptin.

To detect Rat Leptin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat Leptin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect Rat Leptin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat Leptin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.