Leo1 Antibody

• Catalog Number: orb1530587
• Category: Antibodies
• Description: Leo1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 550 and 600
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:10,000 - 1:25,000; IP - 2 - 10 µg/mg lysate; IHC - 1:2,000 - 1:10,000. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Leo1
• UniProt ID: Q8WVC0
• NCBI: NP_620147.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: RDL; replicative senescence down-regulated leo1-li
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Leo1 by western blot.Samples: Whole cell lysate from HeLa, MCF-7, and Jurkat cells. Antibody: Affinity purified rabbit anti-Leo1 antibody orb1530587 at 0.04 µg/ml.

Detection of mouse Leo1 by western blot.Samples: Whole cell lysate from TCMK-1 and NIH 3T3 cells. Antibody: Affinity purified rabbit anti-Leo1 antibody orb1530587 at 0.04 µg/ml.

Detection of mouse Leo1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-Leo1 used at 1:5, 000 (0.2µg/ml).

Detection of human Leo1 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-Leo1 used at 1:5, 000 (0.2µg/ml).

Detection of human Leo1 by western blot of immunoprecipitates.Samples: Whole cell lysate from HeLa cells. Antibodies: Affinity purified rabbit anti-Leo1 antibody.