LDB3 monoclonal antibody (M06), clone 3C8

• Catalog Number: orb2291435
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a full-length recombinant LDB3.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3C8
• Tested applications: ELISA, IF, WB
• Reactivity: Human
• Isotype: IgG2b Kappa
• Immunogen: LDB3 (AAH10929, 1 a.a. ~ 283 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Protein Sequence: MSYSVTLTGPGPWGFRLQGGKDFNMPLTISRITPGSKAAQSQLSQGDLVVAIDGVNTDTMTHLEAQNKIKSASYNLSLTLQKSKRPIPISTTAPPVQTPLPVIPHQKVVVNSPANADYQERFNPSALKDSALSTHKPIEVKGLGGKATIIHAQYNTPISMYSQDAIMDAIAGQAQAQGSDFSGSLPIKDLAVDSASPVYQAVIKSQNKPEDEADEWARRSSNLQSRSFRILAQMTGTEFMQDPDEEALRRSRERFETERNSPRFAKLRNWHHGLSAQILNVKS
• NCBI: AAH10929
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Detection limit for recombinant GST tagged LDB3 is approximately 0.03 ng/ml as a capture antibody.

Immunofluorescence of monoclonal antibody to LDB3 on A-431 cell. [antibody concentration 10 ug/ml]

LDB3 monoclonal antibody (M06), clone 3C8 Western Blot analysis of LDB3 expression in A-431.

Western Blot analysis of LDB3 expression in transfected 293T cell line by LDB3 monoclonal antibody (M06), clone 3C8. Lane 1: LDB3 transfected lysate(31 KDa). Lane 2: Non-transfected lysate.

Western blot analysis of LDB3 over-expressed 293 cell line, cotransfected with LDB3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with LDB3 monoclonal antibody (M06), clone 3C8 (Cat # orb2291435). GAPDH (36.1 kDa) used as specificity and loading control.

Western Blot detection against Immunogen (56.87 KDa).