LCK (phospho-Y393) antibody

• Catalog Number: orb214180
• Category: Antibodies
• Description: Rabbit polyclonal antibody to LCK
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse, Porcine, Rat, Sheep
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y393 of human LCK protein. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1-1000, IF/ICC: 1-100-1-500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: LCK
• Entrez: 313050, 3932, 16818
• UniProt ID: P06240, Q01621, P06239
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti Tyrosine-protein kinase Lck antibody, anti Le
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of LCK (Phospho-Y393) expression in Myla2059 (A), HuT78 (B) whole cell lysates. (Predicted band size: 58 kD; Observed band size: 56 kD)

Immunofluorescent analysis of LCK (Phospho-Y393) staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Direct ELISA antibody dose-response curve using Anti-LCK (Phospho-Y393) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.