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Catalog Number | orb1525109 |
---|---|
Category | Antibodies |
Description | LASP1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP, WB |
Reactivity | Human |
Immunogen | between 100 and 150 |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | LASP1 |
UniProt ID | Q14847 |
NCBI | NP_006139.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | LIM and SH3 domain protein 1; MLN50; metastatic ly Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human LASP1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-LASP1 antibody orb1525109 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. LASP1 was also immunoprecipitated by rabbit anti-LASP1 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Detection of human LASP1 by immunohistochemistry. Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-LASP1 Cat. No. orb1525109 used at a dilution of 1: 1, 000 (1µg/ml) (left) and Cat. No. orb1525108 used at a dilution of 1: 200 (1µg/ml) (right). Detection: DAB. Counterstain: Hematoxylin (blue).
ELISA, IF, IHC, IP, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IHC, WB | |
Hamster | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, WB | |
Canine, Human, Mouse, Porcine, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
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