LARP1 Antibody

• Catalog Number: orb1527241
• Category: Antibodies
• Description: LARP1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: between 275 and 325
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: LARP1
• UniProt ID: Q6PKG0
• NCBI: NP_291029.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: La ribonucleoprotein domain family member 1; LARP;
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human LARP1 by western blot. Samples: Whole cell lysate (10 µg) from HeLa, HEK293T, Jurkat, Hep-G2, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-LARP1 antibody (orb1527241) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Detection of human LARP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-LARP1 antibody (orb1527241) used for IP at 6 µg per reaction. LARP1 was also immunoprecipitated by a previous lot of this antibody (orb1527241) and a second antibody against a different epitope of LARP1.

Detection of human LARP1 by immunohistochemistry.Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-LARP1 (orb1527241) used at a dilution of 1: 200 (1µg/ml). Detection: DAB