LAMP2 / CD107b, monoclonal

• Catalog Number: orb1973123
• Category: Antibodies
• Description: LAMP2 / CD107b, monoclonal
• Target: LAMP2 / CD107b, monoclonal
• Clonality: Monoclonal
• Species/Host: Mouse
• Conjugation: Unconjugated
• Reactivity: Human
• Tested applications: IF, IHC, WB
• Dilution range: Western Blot 1:500-1:1000 Immunofluorescence 1:100-1:300 Immunohistochemistry (Paraffin, Frozen) 1:50-1:150
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
• Note: For research use only

Immunohistochemical analysis of paraffin embedded mouse tissue labeling LAMP2/CD107b with orb1973123 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling LAMP2/CD107b with orb1973123 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling LAMP2/CD107b with orb1973123 at 1/100.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1973123 anti-LAMP2/CD107b antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Anti-LAMP2/CD107b monoclonal antibody at 1/500 dilution, Lysates at 50 μg per lane, This blot was produced using a 12% SDS-PAGE. The Nitrocellulose membrane was then blocked for an hour before being incubated with orb1973123 overnight at 4°C.

Human acute myeloid leukemia cell line KG1a was fixed, permeabilized, and stained with LAMP2/CD107b monoclonal antibody orb1973123: 1-50.