Laminin/Lamc1/Lamc2/Lamc3 Antibody

• Catalog Number: orb215896
• Category: Antibodies
• Description: Laminin/Lamc1/Lamc2/Lamc3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IHC-Fr, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: Peptide mixture of laminin gamma1,2,3 (DLNKLNEIEGTLNKAKDEMK; MSELEERARQQRGHLHLLET; LQRKLSLLEQESQQQELQIQ). Laminin gamma has only three subtypes of antibody to gamma1-3 reactive with all isoforms of laminin.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 177298 MW
• UniProt ID: P02468
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Laminin subunit gamma-1;Laminin B2 chain;Laminin-1
• Note: For research use only
• Application notes: WB: The detection limit for Laminin is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of Laminin using anti-Laminin antibody.Lane 1:human HeLa cell; 2:human HepG2 cell.

IHC analysis of Laminin using anti-Laminin antibody. Laminin was detected in a paraffin-embedded section of human lung cancer tissue.

IHC analysis of Laminin using anti-Laminin antibody.Laminin was detected in frozen section of human placenta tissues.