Laminin gamma 1 Antibody / LAMC1

• Catalog Number: orb2637277
• Category: Antibodies
• Description: Laminins are large hetero-trimeric, non-collagenous glycoproteins composed of alpha, beta, and gamma chains. This MAb reacts with laminin B2/1 chain of ~210 kDa and does not cross-react with other basement membrane components or fibronectin.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: LAMC1/3162
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Murine EHS laminin preparation was used as the immunogen for the Laminin gamma 1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Laminin gamma 1 antibody is available for research use only.
• UniProt ID: P11047
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Laminin gamma 1 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using Laminin gamma 1 antibody (clone LAMC1/3162). These results demonstrate the foremost specificity of the LAMC1/3162 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

IHC staining of FFPE human kidney tissue with Laminin gamma 1 antibody (clone LAMC1/3162). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.