Lamin B1 Mouse Monoclonal Antibody (Nuclear Loading Control)

SKU: orb499788

Description

Lamin B1 Mouse Monoclonal Antibody (Nuclear Loading Control)

Research Area

Cell Biology, Lamins, Nuclear envelope

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:1000-5000, IHC-P=1:200-1000, IHC-F=1:200-1000, IF=1:200-1000
• Reactivity: Human, Rat
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 9C11
• Immunogen: Recombinant human Lamin B Protein (314-386/586aa)
• Target: LMNB1
• Molecular Weight: 64 kDa
• Purification: Affinity purified by Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

LMNB1_HUMAN; Lamin-B1; LMNB1; LMN2; LMNB; LMN; ADLD; MCPH26;

Sample: Lane 1: Human Molt-4 cell lysates, Lane 2: Human Hela cell lysates, Lane 3: Human U-2os cell ysates, Primary: Anti-Lamin B (orb499788) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 64 kDa, Observed band size: 64 kDa.

Sample: Lane 1: Rat Pancreas tissue lysates, Lane 2: Rat Testis tissue lysates, Lane 3: Rat Thymus tissue lysates, Lane 4: Human Jurkat cell lysates, Lane 5: Human Molt-4 cell lysates, Lane 6: Human Hela cell lysates, Lane 7: Human U-2os cell ysates, Lane 8: Human THP-1 cell lysates, Primary: Anti-Lamin B (orb499788) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 64 kDa, Observed band size: 64 kDa.

Sample: MOLT-4 Cell (Human) Lysate at 40 ug, Primary: Anti-LaminB (orb499788) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 64kD, Observed band size: 64kD.

Quick Database Links

Gene Symbol

LMNB1

UniProt

P20700