Lamin B Receptor/LBR Antibody

• Catalog Number: orb546307
• Category: Antibodies
• Description: Lamin B Receptor/LBR Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Lamin B Receptor/LBR recombinant protein (Position: H102-F209).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells Direct ELISA, 0.1-0.5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 65-71 kDa
• UniProt ID: Q14739
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Lamin-B receptor; Integral nuclear envelope inner
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U20S cells using anti-LBR antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of LBR using anti-LBR antibody.Lane 1:human placenta tissue;2:human HL-60 cell;3:human K562 cell;4:human THP-1 cell;5:human HEK293 cell;6:human U2OS cell;7;human U-937 cell;8;human Caco-2 cell.

WB analysis of LBR using anti-LBR antibody.Lane 1:rat thymus tissue;2:rat spleen tissue;3:rat testicular tissue;4:mouse thymus tissue;5:mouse stomach tissue;6:mouse testicular tissue;7:mouse SP20 cell.

IF analysis of LBR using anti-LBR antibody. LBR was detected in immunocytochemical section of U20S cells.

IHC analysis of LBR using anti-LBR antibody.LBR was detected in paraffin-embedded section of human lung cancer tissues.