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Item 1 of 8
Item 1 of 8
LAG3 Detection Set (Risk Free)
Catalog Number: orb1227438
| Catalog Number | orb1227438 |
|---|---|
| Category | Assays and Kits |
| Description | LAG3 Detection Set (Risk Free) |
| Species/Host | Mouse |
| Clonality | Monoclonal |
| Tested applications | ELISA, FC, ICC, IF, IHC-P, WB |
| Reactivity | Human |
| Isotype | IgG1, IgG2a |
| Immunogen | LAG3 antibodies were raised against the extracellular domain of human LAG3. |
| Concentration | Antibody 1 mg/mL |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| Storage | Antibodies can be stored at 4˚°C for three months and -20˚°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Buffer/Preservatives | PBS containing 0.02% sodium azide. |
| Note | For research use only |
| Expiration Date | Please enquire. |
Titration curve analysis of LAG-3 mAbs to detect recombinant LAG-3 in ELISA with orb1239547, orb1239545, orb1239551, orb1239549, orb1239550, orb1239548, and orb1239546 antibodies at decreasing concentrations.
Western blot analysis of LAG-3 in over expressing HEK293 cells using orb1239547, orb1239548, and orb1239546 antibodies at (A) 0.25 (B) 0.5 and (C) 1 μg/ml.
Immunocytochemistry of LAG-3 in over expressing HEK293 cells using (A) orb1239547, (B) orb1239545, (C) orb1239551, (D) orb1239549, (E) orb1239550, (F) orb1239548, (G) orb1239546, and (H) control mouse IgG antibody at 1 μg/ml.
Immunofluorescence of LAG-3 in over expressing HEK293 cells using (A) orb1239547, (B) orb1239545, (C) orb1239551, (D) orb1239549, (E) orb1239550, (F) orb1239548, (G) orb1239546, and (H) control mouse IgG antibody at 2 μg/ml.
Immunofluorescence of LAG-3 in human spleen tissue using (A) orb1239547, (B) orb1239551, (C) orb1239549, (D) orb1239550, (E) orb1239548, (F) orb1239546, and (G) control mouse IgG antibody at 10 μg/ml.
Immunohistochemistry of LAG-3 in human lymphoma tissue using (A) orb1239547, (B) orb1239551, (C) orb1239549, (D) orb1239550, (E) orb1239548, (F) orb1239546, and (G) control mouse IgG antibody at 5 μg/ml.
Flow cytometry analysis of LAG-3 over expressing HEK293 cells using (A) orb1239547, (B) orb1239545, (C) orb1239551, (D) orb1239549, (E) orb1239550, (F) orb1239548, (G) orb1239546, and (H) control mouse IgG antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: LAG-3 over expressing HEK293 cells.
A sandwich ELISA was performed using the anti-LAG3 mAbs orb1239547, orb1239545, orb1239551, orb1239549, orb1239550 and orb1239548 as the capture antibodies for the LAG3 extracellular domain antigen with biotin-labeled Risk-Free anti-LAG3 mAbs as the detection antibodies.
