LAG3 Detection Set (Risk Free)

SKU: orb1227438

Description

LAG3 Detection Set (Risk Free)

Images & Validation

• Tested Applications: ELISA, FC, ICC, IF, IHC-P, WB

Key Properties

• Reactivity: Human
• Assay Type: Detection
• Concentration: Antibody 1 mg/mL
• Conjugation: Unconjugated

Storage & Handling

• Storage: Antibodies can be stored at 4˚°C for three months and -20˚°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Disclaimer: For research use only

Titration curve analysis of LAG-3 mAbs to detect recombinant LAG-3 in ELISA with orb1239547, orb1239545, orb1239551, orb1239549, orb1239550, orb1239548, and orb1239546 antibodies at decreasing concentrations.

Western blot analysis of LAG-3 in over expressing HEK293 cells using orb1239547, orb1239548, and orb1239546 antibodies at (A) 0.25 (B) 0.5 and (C) 1 μg/ml.

Immunocytochemistry of LAG-3 in over expressing HEK293 cells using (A) orb1239547, (B) orb1239545, (C) orb1239551, (D) orb1239549, (E) orb1239550, (F) orb1239548, (G) orb1239546, and (H) control mouse IgG antibody at 1 μg/ml.

Immunofluorescence of LAG-3 in over expressing HEK293 cells using (A) orb1239547, (B) orb1239545, (C) orb1239551, (D) orb1239549, (E) orb1239550, (F) orb1239548, (G) orb1239546, and (H) control mouse IgG antibody at 2 μg/ml.

Immunofluorescence of LAG-3 in human spleen tissue using (A) orb1239547, (B) orb1239551, (C) orb1239549, (D) orb1239550, (E) orb1239548, (F) orb1239546, and (G) control mouse IgG antibody at 10 μg/ml.

Immunohistochemistry of LAG-3 in human lymphoma tissue using (A) orb1239547, (B) orb1239551, (C) orb1239549, (D) orb1239550, (E) orb1239548, (F) orb1239546, and (G) control mouse IgG antibody at 5 μg/ml.

Flow cytometry analysis of LAG-3 over expressing HEK293 cells using (A) orb1239547, (B) orb1239545, (C) orb1239551, (D) orb1239549, (E) orb1239550, (F) orb1239548, (G) orb1239546, and (H) control mouse IgG antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: LAG-3 over expressing HEK293 cells.

A sandwich ELISA was performed using the anti-LAG3 mAbs orb1239547, orb1239545, orb1239551, orb1239549, orb1239550 and orb1239548 as the capture antibodies for the LAG3 extracellular domain antigen with biotin-labeled Risk-Free anti-LAG3 mAbs as the detection antibodies.