LAG3 Detection Set

SKU: orb1227426

Description

LAG3 Detection Set

Research Area

Immunology & Inflammation; Cell Biology

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC-P

Key Properties

• Reactivity: Human
• Assay Type: Detection
• Concentration: Antibody 1 mg/mL
• Conjugation: Unconjugated

Storage & Handling

• Storage: LAG3 antibodies should be stored in working aliquots at -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Titration ELISA analysis of LAG-3 sdAbs to detect recombinant LAG-3 (extracellular domain) coated at 1 μg/mL. sdAbs are detected with a mouse mAb against a C-terminal myc-tag followed by a goat anti-mouse IgG-HRP conjugate. orb1274185, orb1274187, orb1274184, orb1274186, orb1238048, and negative control sdAb 1H4 at decreasing concentrations.

Immunofluorescence of LAG-3 in over expressing HEK293 cells using (A) orb1274185, (B) orb1274187, (C) orb1274184, (D) orb1274186, (E) orb1238048, and (F) orb1237001 negative control sdAb. Single domain antibodies at 5 μg/ml.

Immunohistochemistry of LAG-3 in human lymph node tissue using (A) orb1274185, (B) orb1274187, (C) orb1274184, (D) orb1274186, (E) orb1238048, and (F) orb1237001 negative control sdAb. Single domain antibodies at 1 μg/ml.

Flow cytometry analysis of LAG-3 in over expressing HEK293 cells using (A) orb1274185, (B) orb1274187, (C) orb1274184, (D) orb1274186, (E) orb1238048, and (F) Negative control orb1237001. Single domain antibodies at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: LAG-3 over expressing HEK293 cells.