Lag3 antibody

• Catalog Number: orb669762
• Category: Antibodies
• Description: Mouse monoclonal antibody to Lag3
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: C9B7W
• Tested applications: Blocking, FC, IP
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: This antibody was raised by immunising Lewis rats with a murine LAG-3:Ig fusion protein.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: LAG-3
• UniProt ID: Q61790
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD223; LAG3; Lymphocyte activation gene 3 protein
• Note: For research use only
• Application notes: This antibody has been used in flow cytometric analysis of splenocytes (Workman et al, 2005), T cell hybridomas expressing wild‐type or chimeric CD4:murine LAG-3 (Workman et al, 2002) and CD4+ regulatory T cells (Chien et al, 2017). It has also been used in immunoprecipitation analysis of murine T hybridoma 3A9 cells transduced with murine LAG-3 (Li et al, 2004). This antibody blocks LAG-3 function in vitro, inhibiting antigen‐induced IL‐2 production in LAG-3-expressing T cell hybridomas, but does not block LAG-3:MHC class II interaction (Workman et al, 2002). Treatment with this antibody also blocks LAG-3 function in vivo, leading to accelerated homeostatic expansion of WT T cells (Workman et al, 2005). Administration of this LAG-3-blocking antibody, in combination with PD-L1 blockade, synergistically improves the survival of lymphodepleted myeloma-bearing mice (Jing et al, 2015).
• Expiration Date: 12 months from date of receipt.