LAG3 Antibody

• Catalog Number: orb1263554
• Category: Antibodies
• Description: LAG3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This LAG3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-132 amino acids from the Central region of human LAG3.
• Concentration: batch dependent
• Dilution range: For IHC-P starting dilution is: 1:25For FACS starting dilution is: 1:25For WB starting dilution is: 1:1000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 57 kDa
• Target: LAG3
• UniProt ID: P18627
• NCBI: P18627
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Lymphocyte activation gene 3 protein, LAG-3, Prote
• Note: For research use only
• Application notes: For IHC-P starting dilution is: 1:25For FACS starting dilution is: 1:25For WB starting dilution is: 1:1000
• Expiration Date: 12 months from date of receipt.

Antibody staining LAG3 in human thymus tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Overlay histogram showing Jurkat cells stained with Antibody (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit lgG (H + L) at 1/400 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

Western Blot at 1:1000 dilution Lane 1: human ovary lysate Lane 2: mouse liver lysate Lysates/proteins at 20 ug per lane.

Western Blot at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: mouse heart lysate Lysates/proteins at 20 ug per lane.

Western Blot at 1:2000 dilution + 293T/17 whole cell lysate Lysates/proteins at 20 ug per lane.

Western Blot at 1:2000 dilution + mouse heart lysate Lysates/proteins at 20 ug per lane.

Western Blot at 1:2000 dilution Lane 1: K562 whole cell lysates Lane 2: human liver lysates Lane 3: human ovary lysates Lysates/proteins at 20 ug per lane.