Lactoperoxidase antibody (Biotin)

• Catalog Number: orb345053
• Category: Antibodies
• Description: Lactoperoxidase antibody (Biotin)
• Species/Host: Sheep
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, IP, WB
• Reactivity: Bovine
• Isotype: IgG
• Immunogen: Lactoperoxidase [Bovine Milk]
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:500 - 1:2,000, IP: 1:100, WB: 1:50 - 1:500
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Lactoperoxidase (Bovine Milk) (Sheep) Antibody Biotin Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Sheep Serum as well as purified and partially purified Lactoperoxidase [Bovine Milk]. Cross reactivity against Lactoperoxidase from other sources is unknown.
• Conjugation: Biotin
• UniProt ID: P80025
• NCBI: NP_776358.1
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: sheep anti-Lactoperoxidase biotin conjugated Antib
• Note: For research use only
• Application notes: Anti-Lactoperoxidase Biotin Conjugated Antibody has been tested by Dot blot and western blot and is suitable to be assayed against 1.0 ug of Lactoperoxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:6,000 of the reconstitution concentration is suggested for Anti-Lactoperoxidase.
• Expiration Date: 12 months from date of receipt.